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The gestation period of Roborovski Hamsters is 23-30 days and if mating was observed then the time of the expected arrival of the litter can be roughly calculated. If mating was not observed then it is often not possible to know tell when the litter is expected. Many females do not appear pregnant until a couple of days before the birth but an increased aggressiveness of the female towards her mate, colony hamsters or humans is often an indication of pregnancy. The female may banish the male or other hamsters from the nest a couple of days before pregnancy and/or after the birth. As soon as it is noticed the female is pregnant it is best to clean the cage in preparation for the birth.
It is not necessary to remove the male when the female is noticed to be pregnant. However, the hamsters will often mate immediately after birth so if it is not intended to breed further litters the male should be removed prior to the birth.
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